Figure 6
S17 induced apoptosis is related with elevating ROS level in gastric cancer cells. (A) MTT assay demonstrated the effect of NAC (5 mM) on S17-induced cell death of MGC803, HGC27 and SGC7901cells at 48 h. **p < 0.01 vs untreated group. (B) Flow Cytometric analysis showed the effects of NAC (5 mM) on S17(10 μM)-induced cell apoptosis of MGC803 cells. (C) Western blotting demonstrated the effect of NAC (5 mM) on S17(10 μM)-induced protein expression changes at 48 h. (D) Flow Cytometric analysis showed the effect of NAC (5 mM) on S17(10 μM)-induced loss of membrane potential (ΔΨ) in MGC803 cell mitochondria at 48 h. (E) MTT assay demonstrated the effect of different ROS inhibitors (CAT (250 μM), BHA (15 μM), GKT (200 μM), ROE (125 nM), NEO (200 μM) and APO (10 μM)) on S17 (10 μM)-induced cell death of MGC803 at 48 h. **p < 0.01 vs S17 treatment group.
