Figure 1

iNSCs express Nestin and Sox1 and differentiate to neurons, astrocytes, and oligodendrocytes in vitro. Flow cytometry demonstrated that 94.8% of iNSCs co-expressed the NSC markers Sox1 and Nestin (B), while the secondary antibody control was negative for these markers (A). Immunocytochemistry of iNSCs confirmed the co-expression of Sox1 (C) and Nestin (D); co-localization of Sox1 and Nestin (E)). iNSCs underwent spontaneous differentiation in vitro and were stained for mature neural markers 2 weeks and 4 weeks after bFGF withdrawal. Differentiated iNSCs robustly expressed the neuron markers Tuj1 (F) and MAP2 (G; co-localization of Tuj1 and MAP2 (H)), the astrocyte marker GFAP (I) and the oligodendrocyte marker Olig2 (J,K). Panel K is the inset represented by the white dotted square in Panel J. RFI = relative fluorescence intensity.