Figure 6
iNSC Treatment Aids in the Protection of Neurons and Reduces Microglia Activation at the Lesion Border. Quantification of NeuN+ cells/mm2 at the lesion border shows significant reduction of neurons in non-treated animals but no significant change between normal controls and iNSC treated animals (A). Representative image of NeuN+ neurons at the lesion border in normal control (B), non-treated (C), and iNSC treated (D) animals. Quantification of the percentage Iba1+ area at the lesion border shows a significant increase in Iba1+ area in stroked animals compared to normal control animals and a significant reduction in Iba1+ area in iNSC treated animals compared to non-treated animals (E). Representative image of Iba1+ microglia at the lesion border in normal control (F), non-treated (G), and iNSC treated (H) animals. Quantification of the percentage GFAP+ area demonstrates a significant increase in GFAP+ area in both non-treated and iNSC treated groups compared to normal control animals (I). Representative image of GFAP+ astrocytes at the lesion border in normal control (J), non-treated (K), and iNSC treated (L) animals. Data are expressed as mean ± s.d. *Indicates significance from normal controls (p < 0.05). Data is generated from one brain section from each animal (n = 4 per treatment group). #Indicates significance between treatment groups (p < 0.05). Scale bars, B-D, F-H, J-L, 500 μm.
