Table 2 Multiple stepwise regression analysis for independent factor effects on β-glucosidase V max and K m in both potential and in vitro (pure enzyme) extracts using either all, surface or deep peat datasets.
From: Hydrological legacy determines the type of enzyme inhibition in a peatlands chronosequence
Dataset | Kinetic parameter | H+ | H2O | SOM | Phenolics | E4/E6 | DOC | SUVA254 | 540 | CO2 | Adi R 2 | P value | F |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
All | V max | 11.238 (0.520) | 9.225 (0.268) | 91.416 (0.342) | 0.293 | 0.001 | 9.684 | ||||||
K m | 2323217 (0.316) | 192.74 (0.864) | −24.541 (−0.338) | 0.660 | 0.001 | 41.748 | |||||||
In vitro V max | 0.116 (0.889) | −0.085 (−0.537) | 0.316 | 0.001 | 15.559 | ||||||||
In vitro K m | 2237202 (0.462) | 16.85 (0.223) | 34.290 (0.234) | −64.44 (−0.275) | 0.532 | 0.001 | 18.915 | ||||||
Surface | V max | 122.79 (0.493) | 0.217 | 0.004 | 9.615 | ||||||||
K m | 38.20 (0.419) | 281.64 (0.415) | 0.355 | 0.001 | 9.521 | ||||||||
In vitro V max | −0.028 (−0.566) | 0.297 | 0.001 | 14.107 | |||||||||
In vitro K m | n.s. | ||||||||||||
Deep | V max | 410835 (0.651) | 9.088 (0.479) | 1.150 (0.291) | 0.707 | 0.001 | 25.954 | ||||||
K m | 3311465 (0.411) | 41.76 (0.269) | 143.85 (0.594) | 0.687 | 0.001 | 23.696 | |||||||
In vitro V max | +1815 (0.374) | −0.186 (−0.386) | 0.331 | 0.001 | 8.683 | ||||||||
In vitro K m | 3992021 (0.767) | 37.08 (0.370) | 0.456 | 0.001 | 13.986 |
