Figure 4

(A) General scheme of the polymerase-inhibited, toehold-based DNA strand displacement strategy. (B) Fluorescence spectra changes observed upon treatment of the sensing module with variable concentrations of polymerase: (a) 0, (b) 1 × 10−5, (c) 1 × 10−4, (d) 1 × 10−3, (e) 0.01, and (f) 0.1 units, [F4] = [Q5] = [I6] = 50 nM. (C) General scheme of the polymerase-based keypad lock system. (D) Fluorescence intensities of the system at 520 nm with respect to 24 kinds of input sequences.