Figure 2
From: A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis
Glucocorticoid-dependent regulation of sST2 expression. Production of (A) sST2 and (B) aST2L mRNA in HMC-1 cells after treatment for 6 hours with 0–1000 nM Dex, as detected by qPCR (mRNA content normalised to 18 S rRNA). n = 5. *p < 0.05; **p < 0.01. (C) sST2 production by HMC-1 cells exposed to 100 nM Dex, as detected in culture supernatant by ELISA. n = 4. ***p < 0.001. (D) Surface content of ST2L in HMC-1 cells exposed to 100 nM Dex, as detected by flow cytometry. Mean fluorescence intensity (MFI) was obtained after normalisation to the control condition. n = 3. (E) HMC-1 cells stimulated with 100 nM Dex after treatment with or without (+ or −) a receptor antagonist (RU-486) and collected for sST2 ELISA. n = 3. ***p < 0.001. (F) sST2 mRNA expression in HMC-1 cells transduced with lentiviral vectors carrying hGRα, a mutant hGRα (hGRα-Δ428-490), an irrelevant vector (IV), or non-transduced (mock) and treated with 100 nM Dex (mRNA content normalised to 18 S rRNA and compared with the control). Error bars represent the means ± SEM of 4 independent experiments. Kruskal-Wallis test with Dunn’s multiple comparison post-test for each analysis. *p < 0.05; **p < 0.01.
