Figure 7

Calcium transients in cardiomyocytes treated with AngII. Representative normalized fluorescence (Fluo-4) recordings from cardiomyocytes control (A) or treated with AngII (1 µM) for 1 hr (B) and stimulated with a 5 ms external stimulus (1 Hz), individual points represent the signal for individual frames, acquire at 12 Hz. Below each fluorescence recording the respective line-scan images are shown. Bar graph (mean ± sem) of average maximal amplitude of electrically evoked Ca²⁺ transients (C) or time constant of Ca²⁺ decay (D) in cardiomyocytes control or treated with AngII (1 µM) for 1 hr. Data obtained after fitting individual calcium transients to a single exponential (n = 30–40, from 4 different cardiomyocytes preparations). *p < 0.01 with respect to control. (E) Bar graph (mean ± sem) of fractional shortening. For each bar graph, empty bars represents cardiomyocytes control and hatched bar cardiomyocytes treated with AngII (1 µM) for 1 hr. *p < 0.01 with respect to control.