Figure 5 | Scientific Reports

Figure 5

From: Deep Phospho- and Phosphotyrosine Proteomics Identified Active Kinases and Phosphorylation Networks in Colorectal Cancer Cell Lines Resistant to Cetuximab

Figure 5

The effect of the knockdown of activated kinase candidates on cell proliferation. (a) Viability of HCT116 cells was analyzed following treatment with three siRNAs (#1, #2, and #3) specific to each activated kinase summarized in Table S7. Gray bars show results of siRNAs that had a significant effect (q < 0.05) on cell proliferation. Black bars show results of the control siRNA and siRNAs without a significant effect (q > 0.05). White bars show the results of CDK1 siRNA that was a positive control of the siRNA experiment. Error bars show SDs; N = 3. (b) Cell viability of cetuximab-resistant cells under treatment with two TKIs targeting SRC (KX2-391) and YES1 (SU6656) in a dose-dependent manner. Curve fitting was performed to the data from the growth assay. Error bars show SDs; N = 3.

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