Figure 3
From: Microbial colonization is required for normal neurobehavioral development in zebrafish

Locomotor hyperactivity in axenic larvae relative to conventionalized controls at 10 dpf. Axenic (AX), conventionalized (AC1), and/or conventionally colonized (CC) larvae were subjected to behavioral testing at 6 or 10 dpf. Line graphs showing distance moved (cm) during each 2 min epoch at (A) 6 dpf or (C) 10 dpf. White and black bars along the x axis represent the light and dark periods, respectively. Mean movement during each 10 min light or dark period at (B) 6 or (D) 10 dpf. N = 32–48 larvae per group. Different letters indicate significance (p < 0.05). Axenic (blue), conventionally colonized (red), and conventionalized (green) data are shown. Error represents SEM.