Figure 4
From: Microbial colonization is required for normal neurobehavioral development in zebrafish
Antibiotic treatment of conventionally colonized larvae triggers behavioral hyperactivity at 10 dpf. Conventionally colonized embryos and larvae were exposed to amphotericin B (0.25 µg/ml), kanamycin (5 µg/ml) and ampicillin (100 µg/ml) at 0, 1, 6, 7, 8, and 9 dpf (CC + AB; peach). Conventionally colonized (CC; red), axenic (AX; blue), and conventionalized (AC1; green) larvae were included as controls. At 10 dpf, locomotor activity was measured and the (A) distance moved each two min period or (B) mean distance during the 10 min dark period are shown. Black and white bars represent dark and light periods, respectively. N = 24 larvae per group. Different letters indicate significance (p < 0.05). Error represents SEM.
