Figure 5
From: Microbial colonization is required for normal neurobehavioral development in zebrafish

Developmental, but not acute, microbial colonization is required for normal neurobehavioral development. Axenic embryos/larvae were colonized with water harvested from the fish facility at 1 (AC1), 3 (AC3), 6 (AC6), or 9 (AC9) dpf. An axenic cohort was included as a control. (A) Experimental design. (B) Distance moved each two min period or (C) mean distance during the 10 min dark period are shown. Black and white bars represent dark and light periods, respectively. N = 12–67 larvae per group. Different letters indicate significance (p < 0.05). Error represents SEM.