Figure 7
From: Microbial colonization is required for normal neurobehavioral development in zebrafish
Heat-killed bacteria and microbe-associated molecular patterns are not sufficient to block locomotor hyperactivity in axenic larvae. Axenic larvae were exposed to heat-killed E. coli (HKEB) or S. typhimurium (HKST) (3,125 cells/ml), the bacterial MAMP pam3CSK4 (1.6 ug/ml), or the viral MAMP Poly(I:C) (31.25 ug/ml) at 1, 6, 7, 8, and 9 dpf and subjected to behavioral testing at 10 dpf. Distance moved each 2 min period (cm) in axenic larvae treated with (A) heat-killed bacteria, (C) Pam3CSK4, or (E) Poly(I:C) are shown. (B,D, and F) Mean distance moved (cm) during the 10 min dark period are also shown. Black and white bars represent dark and light periods, respectively. N = 18–48 larvae per group. Different letters indicate significance (p < 0.05). Error represents SEM.
