Figure 1 | Scientific Reports

Figure 1

From: Development of oriC-plasmids for use in Mycoplasma hyorhinis

Figure 1

Prediction of the origin of replication of M. hyorhinis. A 1935-bp large oriC region (LoriC) of M. hyorhinis strain HUB-1 was predicted around the dnaA. It contains short AT-rich regions of 83% and 85% upstream and downstream dnaA respectively. To generate mini-oriC, the regions upstream and downstream dnaA, were amplified as fragment A (563-bp) and fragment B (313-bp). These two fragments were joined by overlapping PCR to form mini-oriC (MoriC, 876-bp).

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