Figure 3

Schematic representation of the procedures for the construction of oriC-plasmids for M. hyorhinis. The tetM was cloned at PstI/SpeI restriction enzymes sites, spiralin gene promoter was cloned at SpeI restriction enzyme site, LoriC and MoriC were cloned at ApaI restriction enzyme site, Hemolysin left arm (LA) was cloned at SpeI restriction enzyme site, Hemolysin right arm (RA) was cloned at SalI restriction enzyme site and finally, the recA spliced with spiralin gene promoter was cloned at NcoI restriction enzyme site. The resultant oriC-plasmids along with their vectors designations were presented.