Figure 4

Prediction of the possible integration of pGEMT-LoriC plasmid containing large oriC (LoriC) at the oriC-region of M. hyorhinis (A). Following integration at the oriC region, a fragment of about 4037-bp could be detected with PCR using integration primers (P10, Table 1): forward integration primer (P10-F, Table 1) binds specifically to M. hyorhinis genome upstream the LoriC, while reverse integration primer (P10-R, Table 1) binds specifically to tetM of the pGEMT-LoriC plasmid (B) to yield a product of predicted size of 4037 bp. The gel image was cropped and full-length gel is included in the Supplementary Fig. S9.