Figure 5
From: Development of oriC-plasmids for use in Mycoplasma hyorhinis
Prediction of the possible insertion of tetM along with spiralin gene promoter at the hemolysin site using Mini-oriC-HT1 and Mini-oriC-HT2 targeting plasmids. Following insertion at hemolysin site, a fragment of about 3072-bp could be amplified with PCR using hlyC flanking primers. Wild-type hlyC exhibits 1566-bp. LA = left arm, RA = right arm.
