Figure 6

Analysis of hlyC disruption: DNA was extracted from the grown culture of M. hyorhinis transformed with Mini-oriC-HT1 (A) and Mini-oriC-HT2 (B) targeting plasmids along with control untransformed cultures, was subjected to PCR analysis using hlyC flanking primers (P11, Table 1) to investigate the integration of the tetM into M. hyorhinis genome at hlyC site. Wild-type hemolysin exhibits 1566-bp while mutant hemolysin that encodes tetM along with spiralin gene promoter has about 3072-bp. (C) the phenotype of the wild-type and mutant colonies of M. hyorhinis. The gel image was cropped and full-length gel is included in the Supplementary Fig. S10.