Figure 4

Disrupted bile acid distribution in liver and gallbladder of cholestasis was regulated by fenofibrate. (a) PCA score plot and loading plot derived from UPLC-QTOFMS data of hepatic ions. Each point represented an individual mouse liver sample (left) and an ion (right). Metabolites were labeled in the loading plot (◾, control group; ▾, ANIT group; ⦁, ANIT+Feno group). Fenofibrate recovered the elevated bile acid levels in liver (b) and gallbladder (c) by ANIT. (d) QPCR analysis of the hepatic gene expression of bile acid synthesis (Cyp7a1 and Cyp8b1), basolateral uptake transporters (Ntcp, Oatp1 and Oatp4), basolateral efflux transporters (Ostβ, Mrp3 and Mrp4), and canalicular transporters (Bsep and Mrp2). Values represented fold change after normalization to control. All data were repressed as mean ± SEM (n = 5). *P < 0.05, **P < 0.01, and ***P < 0.001 verse control; ^# P < 0.05, ^## P < 0.01, and ^### P < 0.001 verse ANIT.