Figure 3
Chilling causes SD in the CNS characterized by a negative shift of extracellular field potential. (A) Representative example of the surge of extracellular potassium [K+]o associated with chilling. (B) Representative example of a field potential (FP) recording from the neuropil of the metathoracic ganglion. The surge in [K+]o and the abrupt negative shift in FP occur simultaneously and are hallmarks of SD. (C) Quantification of the three phases of FP changes during chill-induced SD (positive 1, negative, positive 2). Data represented as mean ± standard deviation. (D) Simultaneous recordings of FP from two locations in the mesothoracic ganglion during chilling. The traces have been scaled to be the same amplitude for display purposes. Note the time lag between SD recorded in the right and left electrodes. (E) Representative example of simultaneous recording of nerve activity in the left metathoracic nerve 5 and FP in the metathoracic ganglion during chilling. Note the bursts of neuronal activity prior to SD and on recovery. (F). Same as E, but in a different preparation to show that peripheral electrical activity could be recorded after SD during chilling.
