Figure 2 | Scientific Reports

Figure 2

From: Immunization with truncated envelope protein of Zika virus induces protective immune response in mice

Figure 2

Immunogenicity of recombinant ZIKV E90 in mice. (A) Schematic diagram with a timeline of mice experiment. Mice were immunized intraperitoneally (i.p.) with ZIKV E90 with aluminum adjuvant and boosted twice every two weeks. Mice sera were collected 35 and 42 days after the first immunization for antibody assays. (B) Indirect immunofluorescence assays were performed by using the sera from the PBS or ZIKV E90-immunized mice. ZIKV-infected Vero cells were incubated with antisera collected at the indicated time points followed by incubation with FITC conjugated goat anti-mouse IgG antibody. (C) The IgG antibody titers against ZIKV and DENV were determined by ELISA, respectively. (D) The neutralization antibody titers against ZIKV and DENV were determined by a standard plaque reduction neutralization assay, respectively. ND: not determined. Dotted line: the detection limits.

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