Figure 1

Expression of TRIB3 during the osteogenic differentiation of hBMSCs. (A) Real-time PCR analysis of TRIB3 mRNA expression in cells induced for 0, 3, 7, and 14 days. (B) Western blot analysis of TRIB3 protein expression in cells induced for 0, 3, 7, and 14 days. (C) Relative expression level of TRIB3 protein quantified and plotted. (D) Expression of TRIB3 determined by immunostaining with anti-TRIB3 at days 0, 3, 7, and 14 of osteogenic differentiation. Red: TRIB3; Blue: DAPI. Scale: 40 μm. *P < 0.05, **P < 0.01.