Figure 2 | Scientific Reports

Figure 2

From: TRIB3 inhibits proliferation and promotes osteogenesis in hBMSCs by regulating the ERK1/2 signaling pathway

Figure 2

Effects of TRIB3 knock-down on the osteogenic differentiation of hBMSCs. (A) Real-time PCR analysis of marker genes specific for osteoblasts, such as ALP, COL1A1, OPN, and OCN, in cells induced for 0, 3, 7, and 14 days. (B) Analysis of ALP activity in cells induced for 0, 3, 7 and 14 days. (C) ALP staining of cells induced for 0, 3, 7 and 14 days. Scale: 100 μm. (D) Mineralization (intense red clusters) in cells induced for 0, 3, 7 and 14 days. Scale: 100 μm. control shRNA: hBMSCs transfected with negative control-shRNA-Vector; TRIB3 shRNA: hBMSCs transfected with TRIB3-shRNA-Vector. *P < 0.05, **P < 0.01.

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