Figure 3

Effects of TRIB3 knock-down on the proliferation of hBMSCs during osteogenic induction. (A) Cell proliferation at days 0, 3, 7 and 14 of osteogenic differentiation detected by EdU assay. Green: EdU dye; Blue: DAPI. Scale: 50 μm. (B) The proliferation rate from days 0 to 14 of osteogenic differentiation was measured using the CCK8 detection kit. control shRNA: hBMSCs transfected with negative control-shRNA-Vector; TRIB3 shRNA: hBMSCs transfected with TRIB3-shRNA-Vector. *P < 0.05, **P < 0.01.