Table 7 Detail of PCR Primers and restriction endonucleases primers.
From: SIRT1 rs10823108 and FOXO1 rs17446614 responsible for genetic susceptibility to diabetic nephropathy
SNP | Location | Chr:position | MAFa | Endonuclease | Primers |
|---|---|---|---|---|---|
rs3818292 A > G | SIRT1,Intron | Chr10:67907144 | G = 0.1272 | VspI | F: GACAATTCCAGCCATCTCT |
R: CTCACGCCTGTAATCCTAG | |||||
rs4746720 T > C | SIRT1,Intron | Chr10:67917073 | C = 0.1156 | Hpy1881 | F: AGGGAACAGCTAATCTAGACCA |
R: AAAGTAAAGACAACCGAGTGCT | |||||
rs10823108 G > A | SIRT1,Intron | Chr10:67900736 | A = 0.1272 | Hpy1881 | F: TCTCAGCCTCCCAAGTAG |
R: AGTAGTAACCAGCAGTCATC | |||||
rs17446614 G > A | FOXO1,Intron | Chr13:40565740 | A = 0.1767 | CviJI | F: CACAGCACCAACACCATAG |
R: GACAGCCTCACCTTAGTATT | |||||
rs2721068 T > C | FOXO1,Intron | Chr13:40565575 | T = 0.4856 | TspRI | F: AGAGCCACTTAATAGGATGAC |
R: ACCTTTGTCTGTCTTCTTGTACAC |
