Figure 4
From: Metal concentrations and distributions in the human olfactory bulb in Parkinson’s disease
Micrographs and heat maps of a single section from case PD1. Note the high levels of aggregated alpha-synuclein in the anterior olfactory nucleus; this region is low in both iron and copper, but high in zinc. (a) Overview of scanned section stained with phosphorylated alpha-synuclein (red), PGP9.5 (green), and Hoechst (blue). Inset shows anatomical detail of the anterior olfactory nucleus and the mitral cell, external plexiform, and glomerular layers in an adjacent section. (b) Trace from (a) showing the distinct layers of the olfactory bulb. Zoom is shown inset. (c–e) Heat maps of localized iron (c), zinc (d), and copper (e) concentrations in the olfactory bulb and tract, with zooms shown inset. OFT: Olfactory tract; ONL: Olfactory nerve layer; AON: Anterior olfactory nucleus; GL: Glomerular layer; EP: External plexiform; MCL: Mitral cell layer; IP: Internal plexiform and granule cell layer. Scale bars: 2 mm (a–e) and 500 µm (a–e inset)
