Figure 1 | Scientific Reports

Figure 1

From: Topological N-glycosylation and site-specific N-glycan sulfation of influenza proteins in the highly expressed H1N1 candidate vaccines

Figure 1

Multiple-stage MS/MS analysis of a hemagglutinin glycopeptide of the doubly charged ion of m/z 1309.5519 by LTQ-FT mass spectrometry. (A) Full MS scan of the PGC fraction at 25% acetonitrile elution of a tryptic digest followed by protease K cleavage. (B) Glycan sequencing by MS/MS at the precursor ion of m/z 1309.55. (C) Peptide sequencing by MS3 fragmentation of the abundant monoglycosylated peptide ion at m/z 906.61. Yellow circle, galactose; blue square, N-acetylglucosamine; green circle, mannose; red triangle, fucose.

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