Figure 1

MS-HRM analysis of DNA methylation. (a) Schematic representation of MS-HRM. Normalized melting curve. Control DNA samples were mixed and adjusted to 0%, 25%, 50%, 65%, 80%, 90%, 95%, and 100% methylated. (b) Normalized difference plot of control DNA samples. Melting data of 0% methylated standard sample was set to baseline (grey). (c) Standard curve of ELOVL2. Error bars represent standard errors. (d) Standard line of EDARADD. Error bars represent standard errors.