Figure 1 | Scientific Reports

Figure 1

From: Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells

Figure 1

SYBR Green I can selectively visualize mt-nucleoids in Fucci2 cells. (a) Schematic representation of the Fucci2 cells used in this study. Average duration of each phase is shown. (b) Time-lapse series of typical fluorescent images during the cell cycle in Fucci2 cells. The duration of S phase was 9 h based on the experiment with EdU pulse labeling (blue bar, the detailed method is presented in the Materials and Methods section). Images showing the color of the nucleus are also presented as merged images. Scale bar, 10 μm. (c) Changes of staining pattern depending on the SYBR Green I concentration in normal HeLa cells. Dilutions of SYBR Green I are indicated above the images. DNAs were immunostained using anti-DNA antibodies in cells without staining of SYBR Green I (panel on the far right). Scale bars, 10 μm. (d) Selective visualization of mt-nucleoids in Fucci2 cells using a low concentration (1:300,000 dilution) of SYBR Green I. The color of the nucleus denoting the cell cycle phase is indicated in the upper left of each image. Scale bar, 10 μm.

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