Figure 5
Predominant increase in the number of mt-nucleoids was observed during the S phase. (a) The number of mt-nucleoids per cell in each cell cycle phase. SYBR Green I-stained mt-nucleoids were counted in Fucci2 cells in each phase. Error bars indicate standard deviation (Early G1 ncells = 19, G1 ncells = 22, Early-middle S ncells = 78, Late S-G2 ncells = 42). (b) The period of time-lapse imaging for the experiment in Fig. 5c. Fucci2 cells were classified into five phases depending on the change in color of their nucleus. (c) Increase in the number of mt-nucleoids within 4 h during the cell cycle. We stained Fucci2 cells with 1:300,000 SYBR Green I, and performed time-lapse imaging at 4-h intervals in each cell cycle phase presented in Fig. 5b, after which we counted the mt-nucleoid number at 0 and 4 h. Error bars indicate standard deviation (ncells = 10 for each phase).
