Figure 1 | Scientific Reports

Figure 1

From: Investigation of Mitochondrial Metabolic Response to Doxorubicin in Prostate Cancer Cells: An NADH, FAD and Tryptophan FLIM Assay

Figure 1

Increase in the enzyme-bound fraction of NAD(P)H-a2% upon doxorubicin treatment in PCa cells. Upper panel (a–f) LNCaP and lower panel (g–l) E006AA (AA) cells. PCa cells: LNCaPs (n = 26) with 4400–4890 ROIs, and E006AA cells (n = 28) with 5585–6372 ROIs, each ROI of 2 × 2 pixels, were analyzed for 5 time points (0, 15, 30, 45 & 60 min). The representative (a–e and g–k) color coded images and (f and l) the histograms showed increase in the enzyme-bound fraction of NAD(P)H-a2% with time (0–60 min) upon treatment with 1 µM doxorubicin when compared with untreated 0 min time point control (Ctrl). Greater magnitude of response was seen in LNCaP vs E006AA cells (60% vs 23%) from 0 to 60 min.

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