Figure 3 | Scientific Reports

Figure 3

From: Investigation of Mitochondrial Metabolic Response to Doxorubicin in Prostate Cancer Cells: An NADH, FAD and Tryptophan FLIM Assay

Figure 3

Increase in mitochondrial NAD(P)H-a2/FAD-a1 Redox Ratio upon doxorubicin treatment in PCa cells. Mitochondrial OXPHOS activity uses NAD(P)H (more enzyme bound-a2%) and produces FAD (less enzyme bound-a1%). The histograms showed increase in mitochondrial NAD(P)H-a2/FAD-a1 Redox Ratio in (a) LNCaP and (b) E006AA (AA) cells for the same ROIs (mentioned above) with time (0–60 min) upon treatment with 1 µM doxorubicin compared with 0 min untreated control (Ctrl). The data suggests that there is increased mitochondrial OXPHOS activity with doxorubicin treatment. Greater magnitude of response was seen in LNCaP vs E006AA cells (89% vs 38%) from 0 to 60 min.

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