Figure 5 | Scientific Reports

Figure 5

From: Investigation of Mitochondrial Metabolic Response to Doxorubicin in Prostate Cancer Cells: An NADH, FAD and Tryptophan FLIM Assay

Figure 5

Increase in mitochondrial ROS upon doxorubicin treatment. LNCaP cells were labeled with MitoSox Red. After imaging of the control cells for 0 min time point, 1 µM doxorubicin was added on stage and proceeded with time lapse imaging at 15 min intervals up to 60 min. Representative images of cells (n = ~100) (a) 0 min control (Ctrl), (b) increased ROS at 60 min doxorubicin treatment, (c) auto-contrasted image with clusters of adjoining cells (n = ~100) grouped together to represent individual ROIs as numbered, (d) were quantified and showed a 1.5-to 5.4-fold increase in ROS over time after doxorubicin treatment.

Back to article page