Figure 9 | Scientific Reports

Figure 9

From: A Novel Benzocoumarin-Stilbene Hybrid as a DNA ligase I inhibitor with in vitro and in vivo anti-tumor activity in breast cancer models

Figure 9

Interaction studies of compound 19 with hLigI and DNA. Fluorescence spectra of (A) hLigI in the presence of compound 19, upon excitation at 280 nm. Fluorescence intensity is indicated in arbitrary units (A.U.). (B) CD spectroscopy shows conformational change in hLigI after addition of compound 19. Spectra was recorded at the wavelength of 250-195 nm. (C) EMSA shows that compound 19 breaks the interaction between DNA and hLigI protein (lanes 3-5). For complementation, increasing concentrations of hLigI, when added back to the reaction, could rescue the hLigI-DNA complex even in the presence of the inhibitor (lanes 6-7). (D) DNase I cleavage assay rules out the possibility of any kind of interaction between compound 19 and DNA. The gel images are representative of three independent experiments.

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