Figure 6 | Scientific Reports

Figure 6

From: Peptide-mediated delivery of donor mitochondria improves mitochondrial function and cell viability in human cybrid cells with the MELAS A3243G mutation

Figure 6

Cell viability and mitochondrial morphology after oxidation induced by hydrogen peroxide. (A) Double staining of live/dead cells with calcein-AM/PI was used to assess cell survival after 2 h treatment with 200 μM hydrogen peroxide. (B) Apoptotic cells quantified by counting PI-positive cells in images at the same magnification. (C) Morphology of mitochondria stained with MitoTracker Red observed by confocal microscopy of surviving cells under oxidative stress. (D) Quantification of tubular and fragmented mitochondria was further analyzed using a semi-automatic system. Images from three independent areas containing approximately 100–150 mitochondria from about 6–8 cells were analyzed from each group (n = 3). Control cybrid cells (Con); MELAS cybrid cells (MELAS); MELAS cybrid cells treated with Pep-1 alone (Pep-1) or Pep-1-labeled mitochondria (Pep-1-Mito). *p < 0.05, compared to control group; + p < 0.05, compared to MELAS group; # p < 0.05, compared to Pep-1 group.

Back to article page