Figure 7

(A) A cartoon schematic shows the mechanism of nuclear entry for IAuPs during mitosis. As cells transition from prophase to metaphase, the nuclear envelope breaks down causing the nuclear and cytosolic contents to mix. The nuclear envelope reassembles in early telophase. (B) Images show asynchronous or S-phase arrested HeLa cells with IAuPs in the nucleus (red arrow), cytosol (yellow arrow) or in a vacuole (green arrow). The inset is a zoomed in region of a cell showing a vacuole, indicated by a dashed line, containing IAuPs. (C) Time-lapse images show dividing HeLa cells treated with citrate-capped AuNPs (top panel) or IAuPs (bottom panel). Yellow arrows indicate AuNPs or IAuPs that segregate to the poles of the cell, while red arrows point to IAuPs that stay near the chromatin and are likely incorporated into the nucleus. The scale bars are 10 μm.