Figure 8
p17 staining localizes to plaque and fibril-like cortical structures in p17-injected mice. Representative immunohistochemistry of brain sections showing the staining for (a) p17 (N-DAB stain, gray-black), (b) β-amyloid (Aβ, DAB stain) and (c) focus on a Aβ plaque in p17-injected cortical regions. (d) Fibril-like structures composed of p17 within the local cortex to injection site and (e) p17-positively stained astrocytes. (f) Lack of Aβ staining in a sham control-injected wild -type mouse and (g) positive staining of Aβ was observed in sham-injected 3xTg-positive mice. (a–g) Scale bar = 50 µm apart from (c) = 10 µm. Images show representative staining results from one or more of six animals in each grouping where sectioning throughout the whole bregma was carried out. See Supplementary Information for immunofluorecence and double immunohistochemistry staining patterns as well as primary antibody-negative controls.
