Figure 1 | Scientific Reports

Figure 1

From: Inducing fluorescence of uranyl acetate as a dual-purpose contrast agent for correlative light-electron microscopy with nanometre precision

Figure 1

Uranyl acetate can be induced to fluoresce at cryogenic temperatures. (A) Emission spectra of uranyl acetate at temperatures between 21 °C and −195 °C. (B) Fluorescence microscopy images of Vero E6 cell sections prepared for TEM. The three sections were first imaged at 21 °C (i) before being cooled down to −195 °C and imaged again (ii), using identical illumination and acquisition parameters. The contrast of each panel is linearly stretched for comparison. (C) Comparing the uranyl acetate fluorescence signal from sections of Vero E6 cells imaged at 21 °C (i) and −195 °C (ii). (D and E) Line traces of the pixel intensities shown in (C i) (red) and (C ii) (blue). Absolute pixel values are shown in (D), and pixel values plotted on separate axes in (E). All scale bars represent 10 µm.

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