Table 1 An overview on the accuracy of alignment for the images shown in Figs 3–6.
Figure | 3 A | 3B | 3 C | 4 | 5 | 6 |
|---|---|---|---|---|---|---|
Number of beads picked for LOOCV | 4* | 20 | 20 | — | — | — |
Accuracy of alignment (LOOCV) | 333 nm | 112 nm | 64 nm | — | — | — |
Number of structures analyzed for uranyl acetate based alignment | 10 | 10 | 10 | 10 | 10 | 10 |
Accuracy of alignment, based on uranyl acetate | 26 nm | 30 nm | 25 nm | 21 nm | 38 nm | 36 nm |
Alignment of FP to uranyl acetate (FP variant in brackets) | — | — | — | 12 nm (GFP) | 24 nm (RFP) | 50 nm (RFP) |
Overall alignment accuracy | 26 nm | 30 nm | 25 nm | 24 nm | 45 nm | 62 nm |
