Figure 3
From: BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts
Dlx3 and Osx stimulate Dspp promoter activity. (A) Schematic illustration of the constructs used in the luciferase (Luc) assay. (B) Luciferase reporter assay showed Dlx3 and Osx enhanced p318 promoter activity, but did not influence the activities of p1318, p591 and pGL3-Basic. The value obtained from the untransfected control group (pGL3-Basic only) was taken as 1-fold, and fold increases were calculated by dividing the individual value by the control group value. *P < 0.05; **P < 0.01; compared with control group. (C) ChIP assay demonstrated the binding of Dlx3 and Osx with Dspp promoter. Neg. IgG: Negative control IgG. Uncropped images of gels were shown in Supplementary Fig. S4.
