Figure 1
From: Epithelial Chloride Transport by CFTR Requires TMEM16A
Intestinal epithelial knockout of TMEM16A eliminates CFTR currents. (a) Original recordings of the transepithelial voltage Vte and the effect of carbachol (CCH, 100 µM) in colonic epithelia from mice with intestinal epithelial knockout of TMEM16A (Vil1-Cre–TMEM16A flox/flox; TMEM16A−/−) and wild-type mice (TMEM16A+/+). (b) Summary of the calculated CCH-induced short circuit currents (ΔIsc). (c,d) Original recordings of Vte and summary of cAMP (IBMX 100 µM/forskolin 2 µM)-induced ΔIsc in +/+ and −/− colonic epithelia. (e,f) cAMP-induced secretion in intestinal organoids from +/+ and −/− intestines (upper panel) and summaries of luminal area increase (lower panel). (g–i) Activation of whole cell currents by CCH and inhibition by CaCCinhAO1 (AO1; 10 µM) in isolated intestinal epithelial cells from +/+ mice. Original recordings (g), individual experiments (h) and current/voltage relationships (i). (j–l) Corresponding experiments in intestinal epithelial cells from −/− mice. (m–o) Activation of whole cell currents by cAMP and inhibition by CFTRinh172 (CFTRinh; 10 µM) in isolated intestinal epithelial cells from +/+ mice. (p–r) Corresponding experiments in intestinal epithelial cells from −/− mice. Mean ± SEM; *Significant activation by cAMP or CCH (paired t-test). #Significant difference between −/− and +/+ (unpaired t-test). (number of mice or cells, respectively).
