Figure 2
From: Modular assembly of a protein nanotriangle using orthogonally interacting coiled coils
Native-PAGE analysis of interactions between linked-SYNZIP fusion proteins after thermal annealing. For lanes 1–6, one or two of constructs 1–6, 5–4 and/or 3–2 (20 µM each) were mixed and annealed. For lanes 7–9, 1–6 and 5–4 were mixed and annealed with varying concentrations of 3–2 (20, 40, or 60 µM). The red arrow indicates the band corresponding to the triangle.
