Figure 1 | Scientific Reports

Figure 1

From: Small molecule T63 suppresses osteoporosis by modulating osteoblast differentiation via BMP and WNT signaling pathways

Figure 1

Identification of T63 by high-throughput screening. (a) Flow chart of the screening procedure. (b) Upper panel: Scatter plot of the first round luciferase-dependent screening in MC3T3-E1-OSE cells. Lower panel: 115 compounds selected for the second round screening. (c and d) RUNX2 transcriptional activities and ALPL activity of the ten hits (n = 3). *p < 0.05, **p < 0.01, **p < 0.001 versus control. (e) Structure of compound T63. (f) EC50 curve and value of T63. MC3T3-E1-OSE cells were treated with different concentrations of T63 for 48 h and luciferase activity was measured. EC50 curve and value was performed by Graphpad prism 5 (n = 3).

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