Figure 3
T63 inhibits the adipogenic differentiation of C3H10T1/2 cells. (a) Representative images of liqid droplets formation. C3H10T1/2 cells were cultured in the adipogenic medium and treated with T63 for 9 days before Oil Red O staining (Scale bar: 50 μm). The area ratios of lipid droplets to nuclei were quantitated. **p < 0.01, ***p < 0.001 versus control (n = 3). (b) Effect of T63 on the mRNA expressions of Pparγ2, Srebf1 and Fabp4. C3H10T1/2 cells were cultured in adipogenic medium in the presence or absence of 5 μM T63 for 6 days before qRT-PCR analysis. CM: complete medium; AM: adipogenic medium. *p < 0.01, **p < 0.01, ***p < 0.001 versus CM control, ## p < 0.01, ### p < 0.001 versus AM control (n = 3).
