Figure 1 | Scientific Reports

Figure 1

From: A fluorescence-based reporter for monitoring expression of mycobacterial cytochrome bd in response to antibacterials and during infection

Figure 1

Construction and basic characterization of the cytochrome bd expression reporter. (A) Genetic construction of the reporter plasmid with the mCherry gene under control of the cydA promoter. (B) Growth of Mycobacterium marinum carrying the cydA reporter in 7H9 medium. Three independent biological replicates were monitored for growth. Error bars indicate the standard deviation (s.d.) value. (C) mCherry fluorescence emitted by the cydA reporter during in vitro culture under aerated (normoxia) conditions and hypoxic conditions. Three independent biological replicates were monitored. The fluorescence signal was corrected for autofluorescence of WT bacteria without a plasmid. Error bars indicate the s.d. (D) cydA induction in response to the nitric oxide donor DETA-NO (1x MIC and 10x MIC) on day 3 after induction. Three independent biological replicates were monitored. Error bars indicate the s.d. value.

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