Figure 3

Differential regulation of cydA in response to antibacterials analyzed by flow cytometry. A histogram plot showing the number of events (y-axis). Peak height indicates mCherry fluorescence intensity (x-axis) for: Untreated samples (red line), 1x MIC of the shown antibiotic (blue line) and 10x MIC (green line). WT without cydA reporter served as a background control (black line). Three independent experiments were performed, each histogram represents data from one representative experiment with 30,000 cells.