Table 1 Information of the pIDM inducible mutants.

pIDM-A11

1;faf1(8, 6^th intron, reverse)

~55%

FDD^c; cartilage defects; DFR^d

pIDM-E10

1;grb14(9, 2^nd intron of isoform X1, 1st intron of isoform X3, reverse)

isoform X1~30%, isoform X3~13%

Curve body, small head, DFR

pIDM-A1

1;wu:fb77a09(17, 9^th intron of isoform X1, reverse)

X1-X7~45%

Short stature; DFR

pIDM-A28

2;[galnt2(13, 1^st intron of galnt2, reverse)]; [N.D]

galnt2~41%

Short stature; DFR

pIDM-A3

3;[sema5ba(9, 15^th intron, reverse)];[plekha5(4, 12^th intron, reverse)];[LOC101882061(23,4^th intron, reverse)]

LOC101882061~38%

DFR

pIDM-A18

2;[ttyh3b(1, 3^rd intron, reverse)];[si:dkey-26i13.8(1,1st exon and ~200 bp upstream ATG, forward)]

ttyh3b~2.5 F; si:dkey-26i13.8~59%

DFR

pIDM-E7

3;[fnip1(21, 16^th intron, reverse)];[cyr61(3, 1st intron, reverse)];[N.D]

cyr61~40%

DFR

pIDM-E3

1;pcyt1αa(2, ~1.0 kb upstream of 1st exon, forward)

~28%

Small eyes with less pigmentation, brain heamorrhage

pIDM-E17

1;ap2b1(5, 19^th intron, reverse)

~44%

Incomplete epiboly, yolk rupture

pIDM-A200

1;sgcd(21, 2^nd intron of sgcd, 2^nd intron of isoformX1, reverse)

~38%

Curve body and pericardial edema, failed heart construction

pIDM-A17

1;LOC556929(23, ~1.7 kb upstream of 1st exon, forward)

~3.3 F^b

Abnormal development with curved body

pIDM-E8

1;plcd1a(24, 1st exon and 75 bp upstream of ATG of isoformX1, forward)

~12 F, isoformX1~11 F

No eyes, small brain and curved body

pIDM-A33

3;[gpr128(22, 64 bp upstream of 1st exon, forward)]; [picalmb(15, 12^th intron, reverse)]; [14.165 kb at 5′ site: Loc1039114889(8, forward)]

gpr128 ~ 3.34 F; picalmb ~ 39.5%

Epidermal blisters

pIDM-E22

2;[sidkey-256k13.2(23, 5′UTR region and ~4.4 kb upstream of ATG, forward)]; [ms4a17a.0.8(4, ~4.5 kb upstream of 1st exon, forward)]

sidkey-256k13.2~ 4.15 F; ms4a17a.0.8~ 6.16 F

Less pigmentation

pIDM-A20

4;[f2rl1.1(21, ~3.6 kb upstream of 1st exon, forward)]; [hes6(2,~2.2 kb downstream of last exon, reverse)]; [myt1l(23, 2^nd intron, forward)];[dner(18, 1st intron, reverse)]

f2rl1.1~3.69 F; hes6,NS;myt1lX1/X2 ~2.19 F; myt1lX3 ~52%; LOC103909182(NC) ~ 2.0 F

Early embryonic lethal

pIDM-E54

2;[syt10(4, 3^th intron, forward)];[N.D]

syt10, NS

Shorter and thicker extension

pIDM-E19

2;[grik4(15,6^th exon, reverse)];[N.D]

grik4-001~76%; grik4-002(NC),NS; grik4-003(NC)~64%; LOC103908836(NC) ~73%

Small head, curved body and unabsorbed yolk

pIDM-E1

3;[LOC103910266(3, 1st intron, reverse)];[113 bp at 3′ side: pim-3(25,reverse)];[si:ch211-15e22.3(1, 7^th, intron, forward)]

syt17 ~84%; LOC103910266(NC) ~41%; si:ch211-15e22.3, NS

Pericardial edema and deformed head

pIDM-E14

2;[abi1a(24, 1st intron, reverse)];[TANC1-like(9, 1st intron, forward)]

abi1a ~39%; acbd5a ~15%;TANC1-like ~37%; LOC103911696(NC) ~34%

Short stature and no pericardium

pIDM-E46

3;[evi5a(2, ~3.5 kb upstream of 1st exon, forward)];[LOC101886424(4, ~2.6 kb upstream of 1st exon, forward)]; [LOC324205 (1, 1st intron, reverse)]

evi5a~4.38F;LOC101886424(NC)~1.97F; LOC324205,NS; tcrig1,NS; LOC101882854-001(NC) ~2.32 F, LOC101882854-002(NC)~1.79 F

Less pigmentation

pIDM-A96

3;[scn8aa(9, 11^th intron, reverse)];[~32.6 kb at 3′ side: LOC568650 (3)]; [~14.3 kb at 3′ side: LOC101884758 (4)]

scn8aa ~39%; tmprss12 ~2.76 F

Arrested development (18–21 somite stage)

pIDM-E256

3;[snx17(20, 1st intron, reverse)];[NLRC3-like(21, 8^th exon, reverse)]; [~3.7 kb at 3′ side: ppp3cca(5, reverse)]

snx17 ~73%; NLRC3-like ~74%

Arrested development (15–26 somite stage)

pIDM-A199

2;[si:ch211-220f12.4(18, 1st intron, reverse)];[ogdhb(10, ~5.5 kb upstream of 1st exon, forward)]

prpf18~2.54 F; ogdhb~5.17 F

Curved body with severe cell death

pIDM-A31

3;[LOC101885416(4, 3rd intron, reverse)]; [lox12a(10, 3rd intron, reverse)]; [~67.7 kb at 3′side: LOC100331306 (13, forward)]

lox12a ~17.6%

Non-specific phenotypes^e

pIDM-E43

3;[tbx1(5,6^th intron, reverse)]; [ablim3(14, 2^nd intron, reverse)]; [dapk1(5,6^th intron, forward)]

tbx1 ~40%; ablim3 ~62%; dakp1, NS

Non-specific phenotypes

pIDM-E55

2;[shisa-7-like(16, 3^th exon, reverse)];[efnb1(5,1st intron, forward)]

shisa-7-like ~44%; efnb1,NS

Non-specific phenotypes

pIDM-E6

7;[ebag9(16,1st intron, forward)];[matn4(6,1st intron, reverse)]; [prdm5(23, 13^th intron, reverse)]; [LOC101883267(13, ~500 bp upstream of 1st exon, forward)]; [ube2nb(4,1st intron, forward)]; [tbc1d8(9, 7^th intron, reverse)];[ifnphi3(3,2^nd intron, reverse)]

ebag9~3.31 F; matn4~55%; prdm5~55%; ube2nb,NS; tbc1d8 ~68%; ifnphi3, NS

Non-specific phenotypes