Figure 2 | Scientific Reports

Figure 2

From: Functional association of cellular microtubules with viral capsid assembly supports efficient hepatitis B virus replication

Figure 2

HBV replication was reduced upon nocodazole treatment. Hep38.7-Tet (A) and HepG2.2.15.7 (B) cells were treated with 1 μM ETV, 0.5, 1, 2.5 and 5 μM nocodazole, or left untreated (“control”) for 6 days. 1 μM staurosporin used as a positive control for exhibiting cell apoptosis were treated to the cells for 24 h. Intracellular HBV DNA (A-a left, B left) were quantified by real time PCR. Nucleocapsid-associated HBV DNA upon treatment with or without compounds for 9 days was also detected by Southern blot (A-a right). MTT assay (A-b left, B right), caspase3/7 assay (A-b center), and trypan blue staining (A-b right) were also shown to quantify cell viability or apoptotic cell death. (C) HepG2 cells transfected with a plasmid encoding different HBV genotypes (A, C, or D) were treated with or without 1 μM ETV or 1, 2.5, 5 and 10 μM nocodazole for 72 h. HBV replication was examined by quantifying nucleocapsid-associated HBV DNA.

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