Figure 6
Interaction of core and tubulin in Hep38.7-Tet cells and Hep38.2-Tet cells. (A) Tubulin (green) in Hep38.7-Tet and Hep38.2-Tet cells were observed as shown in Fig. 4B. (B) Hep38.7-Tet (lanes 2 and 4) and Hep38.2-Tet (lanes 1 and 3) cells were transfected with an expression plasmid for FLAG-core (lanes 3 and 4) or the empty vector (lanes 1 and 2) for 48 h in the presence of tetracycline. These cell lysates were immunoprecipitated with an anti-FLAG antibody (panels a and c) or were recovered without immunoprecipitation (“total”: panels b, d, and e). Tubulin (panels a and b), FLAG-core (panels c and d) and actin (panel e) were detected by immunoblot. Expression levels for tubulin in panels a and b are quantified by densitometry and are shown as relative levels in blue characters below the panels. (C) Capsid formation (upper) as well as the expression for FLAG-core (middle) and actin (lower) in the cells prepared as in (B) were detected.
