Figure 3 | Scientific Reports

Figure 3

From: Synergy of two low-affinity NLSs determines the high avidity of influenza A virus nucleoprotein NP for human importin α isoforms

Figure 3

Crystal structure of ∆IBB-importin α1 bound to NP-NLS2-K. (A) Structure of ∆IBB-importin α1 (gray surface) in complex with NLS2-K (green sticks). (B,C) Left panel: zoom-in window showing an 2Fo – Fc electron density map (displayed as a blue mesh contoured at 1.25σ above background) calculated using all reflections between 15–2.25 Å resolution after omitting the NLS bound at the major (B) and minor (C) NLS-binding sites of importin α. In both cases, the density is overlaid to the NLS final model (the illustration was generated using PyMol58). Right panel: schematic diagram of the interactions NLS2-K (green) makes at the major (B) and minor (C) NLS-binding sites of importin α1 (white). Side chain nitrogen and oxygen atoms are color-colored in blue and red, respectively. Interactions between importin α side chains and the NLS main chain atoms are shown by dashed gray lines.

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