Table 2 Structural alignment of importin α/Kap60 solved in complex with NLSs.

From: Synergy of two low-affinity NLSs determines the high avidity of influenza A virus nucleoprotein NP for human importin α isoforms

NLS type

Minor Binding Site P-1′P0′P1′P2′P3′P4′P5′

 

Major Binding Site P1 P2 P3 P4 P5

PDB id

SV40T-ag

P K K K R K

 

P K K K R K V

1EJL/1BK6*

hPLSCR1-NLS

  

G K I S K HWTGI

1Y2A

hPLSCR4-NLS

S I I R K W N

  

3Q5U

Guα-NLS

G Q K R S F S

  

3ZIN

A89-NLS

L G K R K Y W

  

4B8P**

B54-NLS

L G K R K R H

  

2YNS**

TPX2

K R K H

P V K M I K

 

3KND

C-Myc

K R V K L

P A A K R V K

 

1EE4*

Nucleoplasmin

A V K R P A A

TKKAG

Q A K K K K L D

1EJY/1EE5*

Kap60-IBB

E L R R R R D

TQQVELRKAKRDEA

L A K R R N F

1WA5*

h1NLS

TR K K R K D P

DSDDWSES

N S K E N K ID

4XZR*

h2NLS

T N K R K R E

QISTDNEAKMQIQEEKS

P K K K R K KRSSKANK

4PVZ*

NP-NLS1

SQGT K R S Y EQM

  

4ZDU

NP-NLS2-K

G R K T R

 

GE N G R K T R

5V5O

NP-NLS2-R

G R R T R

 

G R R T R

5V5P

  1. *Denotes yeast importin α (Kap60). **Denotes rice importin α. In all other cases, mammalian importin α was co-crystallized with NLSs.
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